Alcoholic Hepatitis: A Paradigm shift of Prevention and Treatment Analyzed at Cellular level by Histo/Pathological Micrometric Assessment.

Abstract

Objective: To evaluate role of Vitamin C and Naproxen in Alcohol-induced liver toxicity by micrometric technique (hepatocyte count, size and nuclear diameter) in albino rats.
Methodology: This experimental interventional study was conducted at the sheikh Zayed medical college and hospital Rahim yar khan from December 2022 to March 2023. 60 albino rats with equal gender distribution were selected based on the random probability sampling technique and placed in three groups of 20, again with an equal gender distribution (1:1). Group A (positive control) was given purified Ethanol for 10 days., Group B was given Vitamin C and Naproxen
Prophylaxis for 7 days and later intoxicated with Ethanol for 10 days and Group C was simultaneously Administered Vitamin C, Naproxen, and Ethanol for 10 days. The hepatocyte count, size and nuclear diameter of all the groups were recorded by micrometric technique using ocular and stage micrometric scales. Data analysis was conducted using SPSS 24.0 and P-Value of ≤0.05 considered Statistically Significant.
Results: Group A animals experienced decreased number of hepatocyte count, increase in size of cell and decrease in nucleus size; Group B showed similar picture as group “A” but less in intensity and Group C showed nearly normal hepatic architecture with hepatocyte count, size and nuclear size close to normal ranges.
Conclusion: The anti-oxidative and anti-inflammatory effects of Vitamin C and Naproxen demonstrated significant hepatoprotective effects on liver histologic architecture, providing safety from alcohol-induced liver injury.

Keywords: Naproxen, Prophylaxis, liver toxicity, alcoholic liver disease, vitamin C.