Effects of Piperine on Relative Tissue Weight Index of Ovaries and Gonadotropin Levels in Female Albino Rats.

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Published: Nov 2, 2026
DOI: https://doi.org/10.62118/jmmc.v17i1.890

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Amna Mahmood
Assistant Professor at Department of Biochemistry, Islamic International Medical College, Rawalpindi.
Urfa Zaryab Mir
Assistant Professor at Department of Biochemistry, CMH Kharian Medical College
Mehtab Munir
Assistant Professor at Department of Pharmacology, Rawal Institute of Health Sciences.
Samankal Sara
Senior Registrar at Department of Obstetrics and Gynaecology, Rahbar Medical & Dental College/ Punjab Rangers Teaching Hospital.
Rabia Ejaz
Assistant Professor at Department of Anatomy, CMH Kharian Medical College.
Saqib Mansoor
Associate Professor at Department of Anatomy, Niazi Medical & Dental College Sargodha.

Abstract

Objective: To evaluate the effects of piperine on ovarian relative tissue weight index and serum gonadotropin levels in adult female albino rats.
Methodology: This experimental study was conducted at the department of Anatomy, Niazi Medical and Dental Col-lege, Sargodha, over a period of four-month period from September 2025 to December 2025. Thirty adult female Wistar albino rats were divided into three groups (each group 10 rats): Group A (control; normal saline), Group B (piperine 5 mg/kg/day) and Group C (piperine 10 mg/kg/day). Drugs were given orally for 30 days daily. Body and ovarian weights were recorded to calculate RTWI. Serum levels of FSH and LH were quantified by enzyme-linked immunosorbent assay (ELISA). One-way ANOVA with Tukey’s post hoc test were used to measure the group differences.
Results: A significant increase in ovarian Relative Tissue Weight Index (RTWI) was observed after piperine treatment, with the highest values recorded in the 10 mg/kg group (p < 0.001). Serum FSH and LH levels were significantly in-creased in piperine-treated groups, particularly in Group C (p < 0.001).
Conclusion: Piperine induces dose-dependent ovarian toxicity with altered RTWI, structural ovarian damage and signif-icant disruption of gonadotropin secretion. Therefore, prolonged or high-dose piperine exposure can disturb female re-productive function.
Keywords: Follicle-Stimulating Hormone; Luteinizing Hormone; Ovaries; Piperine; Relative Tissue Weight Index.

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How to Cite
Amna Mahmood, Urfa Zaryab Mir, Mehtab Munir, Samankal Sara, Rabia Ejaz, & Saqib Mansoor. (2026). Effects of Piperine on Relative Tissue Weight Index of Ovaries and Gonadotropin Levels in Female Albino Rats. Journal of Muhammad Medical College, 17(1), 83-87. https://doi.org/10.62118/jmmc.v17i1.890
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Vol 17 No 1 (2026): Vol 17 (1) May-October 2026
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Original Article
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