The influence of manganese deficiency during gestation on the emer-gence of adult diseases. An experimental study on mice progeny.

Abstract

Objective: To detect the possible role of Manganese deficiency in the development of adult diseases when exposed in utero.
Methodology: Total 24 adult female BALB/c female mice were divided into two equal size groups: group 01 was provid-ed normal feeding, whereas group 02 received a Manganese-restricted diet. Animals were permitted to mate once de-sired levels were achieved, and pregnancy was assessed by examining vaginal area. The similar dietary regimen was maintained during the entire gestation. Pups were maintained on the same diet until 15 months of age. The behavior and locomotion of animals was kept under observation. At 05- and 15-months different blood parameters were exam-ined. At 15 months age, the pancreas, kidneys and uterine tissue were excised for microscopic analysis.
Results: The group 2 animal group exhibited anomalous behavior and movement characteristics. In comparison to the control group, offspring in group G2 exhibited increased levels of blood glucose, serum insulin and cholesterol. H & E staining demonstrated modifications in the parenchyma of the pancreas and kidneys and were confirmed by immuno-histochemistry. The uterus appeared normal in both groups as indicated by anti-MLH antibody staining.
Conclusion: In utero Manganese deficiency may eventually leads changes of vital organs and play a role in the develop-ment of diseases later in the life.
Key words: Manganese restriction, mice offspring, adult diseases, in-utero.